Neeraj Soni talk at Indian Institute of Technology Mandi

Neeraj Soni, PhD student in Meller lab visited IIT Mandi and presented his PhD work , in which he talked about the feasibility of whole protein identification and sequencing through sub 5nm solid state nanopore.

Neeraj extensively discussed the biophysical characteristics associated with the single-file translocation of whole proteins, underscoring its importance in contrast to traditional methods. Additionally, he delved into inventive strategies for regulating the dynamics of the translocating molecule as it passes through nanopores.

The lab perspective paper on the emerging field of single-molecule protein identification is out on Nature Methods

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

Read the full paper

Shilo Ohayon presents a talk at the: HealthIL - BME Satellite Event: Engineering the Future of Healthcare

Shilo Ohayon presented the lab research and advancement in single-molecule protein identification and quantification technologies. The 10 min talk was aimed at the general audience and reviewed the latest developments in single-molecule sensing, optical sensing using nanotechnologies such as nanopores and submicron channels.

The talk briefly explained the foundations these technologies are based upon and the importance of this technology have on digital health.

In the future, we believe our technology could assist fast and accurate diagnostic in the community and better monitoring of patients.

Press here to watch the talk

Prof. Meller delivered the Joan van der Waals colloquium in Leiden University

On October 26, 2018, Prof. Meller delivered the Joan van der Waals colloquium in the Department of Physics, Leiden University, the Netherlands. The broadly accessed colloquium was entitled “Nanopore bio-sensing: past, present and future” and covered over 20 years of nanopore research towards DNA sequencing as well as recent developments leading to

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Congratulations to Nitinun for the publication of her Chem Society Review paper

Proteins are the structural elements and machinery of cells responsible for a functioning biological architecture and homeostasis. Advances in nanotechnology are catalyzing key breakthroughs in many areas, including the analysis and study of proteins at the single-molecule level. Nanopore sensing is at the forefront of this revolution. This tutorial review, published on October 17, 2018, provides readers a guidebook and reference for detecting and characterizing proteins at the single-molecule level using nanopores. Specifically, the review describes the key materials, nanoscale features, and design requirements of nanopores. It also discusses general design requirements as well as details on the analysis of protein translocation. Finally, the article provides the background necessary to understand current research trends and to encourage the identification of new biomedical applications for protein sensing using nanopores.